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. 2019 Jul 1;18(16):1849–1867. doi: 10.1080/15384101.2019.1629772

Figure 4.

Figure 4.

SNHG6 was highly expressed in HCC cells. (a) SNHG6 was significantly higher expressed in HCC tissues compared with adjacent normal tissues, expression levels were calculated from the gene/β-actin expression ratio (**P< 0.01, compared with a normal group). (b) SNHG6 expression level in one normal liver cell line: HL-7702 and four HCC cell lines: HepG2, Hep3b, HLE and Huh7. (*P< 0.05, **P< 0.01, compared with HL-7702 group) (c) QRT-PCR showed SNHG6 expression levels in HepG2 and Hep3b cell lines were down-regulated by si-SNHG6. (**P< 0.01, compared with a si-NC group, respectively) (d) Cell viability of two HCC cell lines was analyzed by MTT assay. The inhibition of SNHG6 can significantly decrease the cell viability compared with the NC group. (*P< 0.05, compared with a si-NC group, respectively) (e) QRT-PCR showed SNHG6 expression levels in HepG2 and Hep3b cell lines were up-regulated by pcDNA3.1-SNHG6. (**P< 0.01, compared with Vector group, respectively) (f) MTT assay exhibited pcDNA3.1-SNHG6 can significantly increase the cell viability compared with the vector group. (*P< 0.05, **P< 0.01, compared with Vector group, respectively) (g) Down-stream miR-139-5p and SERPINH1 expressions in different transcription groups were detected by QRT-PCR (**P< 0.01, compared with si-NC or Vector group, respectively). Every experiment was performed for 3 times at least.