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. 2019 Jul 1;18(16):1849–1867. doi: 10.1080/15384101.2019.1629772

Figure 9.

Figure 9.

MiR-139-5p can directly target SERPINH1. (a) The binding sites between wild type SERPINH1/mutant SERPINH1 and miR-139-5p. (b) Dual luciferase reporter assay verified that miR-139-5p could target at SERPINH1-wt rather than SERPINH1-mut (**P< 0.01, compared with the miR-NC group) (c) Relative expression level of mRNA SERPINH1 in miR-139-5p mimics group, miR-139-5p inhibitor group and negative control group. The results were determined by qRT-PCR and western blot. (*P< 0.05, compared with NC group) (d) SERPINH1 was significantly higher expressed in HCC tissues compared with adjacent normal tissues, expression levels were calculated from the gene/β-actin expression ratio (**P< 0.01, compared with a normal group). (e) Relative expression level of SERPINH1 in normal liver cell line and four HCC cell lines (*P< 0.05, ** P< 0.01, compared with normal control). Every experiment was performed for 3 times at least.