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. 2019 Aug 2;7:e7428. doi: 10.7717/peerj.7428

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©2019 Chanchay et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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RT-PCR was performed to validate the expression of twenty selected candidate genes proposed to be involved in JH and ecdysone pathways. RT-PCR products of expected sizes were amplified except for lane 20 (Unigenen12798 [Spot]). (A) M: 1 kb DNA ladder; 1: Unigene12481 (Phm, 1,455 bp); 2: Unigene15996 (Sad, 1,422 bp); 3: CL7713 (Shd, 1,479 bp); 4: CL2776 (Cyp18a1, 1,587 bp); 5: CL5335 (Dib, 1,506 bp); 6: Unigene10748 (Sro, 1,110 bp); 7: Unigene12593 (Sro, 1,182 bp); 8: Unigene19505 (FPPP, 915 bp); 9: CL2037 (FPPP, 918 bp); 10: CL8545 (FOHSDR, 765 bp); 11: Unigene12692 (FOHSDR, 750 bp). (B) M: 1 kb DNA ladder; 12: CL9077 (CYP15A1, 1,521 bp); 13: Unigene14277 (JHE, 1,245 bp); 14: Unigene8839 (JHEH, 1,389 bp); 15: Unigene9157 (JHEH, 1,383 bp); 16: CL987 (JHDK, 549 bp); 17: Unigene21984 (ALDH, 1,494 bp); 18: Unigene9200 (JHAMT, 810 bp); 19: Unigene19886 (Spo, 1,455 bp); 20: Unigene12798 (Spot, 2,000 and 750 bp [expected size: 1,476 bp]). (C) M: 1 kb DNA ladder; 21: partial Unigene19886 (Spo, 1,038 bp); 22: partial Unigene12798 (Spot, 1,011 bp).