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. 2019 Aug 2;7:e7429. doi: 10.7717/peerj.7429

Figure 1. Age-related expression of CYP-1 family gene/proteins in livers of male rats.

Figure 1

(A) AhR, (B) Cy1a1, (C) Cyp1a2. Livers from male SD rats at the fetus (−2 d before birth), the neonatal stage (1, 7, and 14 d), and at weanling (21 d), at puberty (28 and 35 d), at adult (60 and 180 d), and at aging (540 and 800 d), were collected to extract RNA, followed by qPCR analysis (n = 4–5 for each time point). *Significantly different from at birth, p < 0.05. For western blot insert, the neonatal (14 d), at weanling (21 d), at puberty (28 d), at adult (60 and 180 d), and at aging (540 and 800 d) were collected to extract protein. Aliquoted proteins (30 µg) were separated on NUPAGE 10% BT gels and the representative western blot was inserted into the figure (n = 3). The molecular weight for CYP1A1 was 55 kD, and β-actin 43 kD.