Fig. 3. TSPAN12 promotes colony formation in OC cells.
(A–D) Stable A2780 and SKOV3 cells from two single clones with TSPAN12 knockout were seeded in 6-well plates (5 × 102 cells/well) in parallel with control group. Two weeks after inoculation, cell colonies were stained by crystal violet (0.5% w/v), and colony numbers were counted. (E and F) Colony formation assay in OVCAR3 and SKOV3 cells with TSPAN12 overexpression were performed following the above protocol (1 × 103 cells/well for inoculation) after treatment with or without CDK inhibitor, AT7519 (Inh, 100 nM) for 72 h. *P < 0.05; **P < 0.01; ****P < 0.0001. Results are representative of three independent experiments.