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. 2019 Jul 20;42(7):557–567. doi: 10.14348/molcells.2019.0015

Fig. 5. TSPAN12 regulates cell cycle in OC cells.

Fig. 5

Fig. 5

(A–H) Cells were treated with serum-free medium for 24 h and then collected at time 0 h, 4 h, 8 h, and 16 h after releasing. Cell cycle progression in A2780, SKOV3 with TSPAN12 knockout were examined by flow cytometry analysis after PI staining. (I–L) OVCAR3 and SKOV3 stable cells with TSPAN12 overexpression were first treated with or without CDK inhibitor, AT7519 (Inh, 100 nM) for 72 h (100 nM), and then evaluated by flow cytometry analysis according to the above protocol. P values between groups were listed above each bar chart. Results are representative of three independent experiments.