Table 1.
GPCR | NMR Labels | Expression System | Membrane Mimetic | ref. |
---|---|---|---|---|
β2AR | 13CH3-Lys | Sf9 | DDM | 116 |
β2AR | 19F-TET | Sf9 | DDM/CHS | 112 |
β2AR | 13CH3-Met | expresSF+ | DDM | 113 |
β2AR | 19F-BTFA | Sf9 | DDM and LMNG | 117 |
β2AR | 13CH3-Met | Sf9 | DDM | 118 |
β2AR | 19F-BTFA | Sf9 | LMNG | 119 |
β2AR | 19F-TET | Sf9 | DDM/CHS | 120 |
β2AR | 13CH3-Met, 2H | expresSF+ | nanodisc | 121 |
β2AR | 19F-BTFMA | Sf9 | DDM | 122 |
β2AR-T4L | 19F-TET | Sf9 | DDM/CHS | 123 |
β2AR | 13CH3-Met, 2H Cterm-2H, 13C, 15N | expresSF+ + E. coli (segmental label) | nanodisc | 124 |
β1AR | 15N-Val | High Five | DM | 114 |
β1AR | 13CH3-Met | Sf9/Sf21 | LMNG | 115 |
BLT2 | 13CH3-Ile | E. coli | nanodisc | 130 |
A2AAR | 19F-BTFMA | P. pastoris | LMNG/CHS | 125 |
A2AAR | 13CH3-Ile, 2H | P. pastoris | DDM | 126 |
A2AAR | u-15N, ~70% 2H | P. pastoris | LMNG/CHS | 127 |
A2AAR | 19F-BTFMA | P. pastoris | LMNG/CHS | 131 |
MOR | 13CH3-Met, 2H | expresSF+ | LMNG/CHS | 128 |
MOR | 13CH3-Lys | Sf9 | LMNG/CHS | 129 |