Table 1.

Summary information for the chemicals used in the nine presented medaka multi-generational tests.

Chemical	Mode of Action	Quantification Method	Test Concentrations (mean ± standard deviation)
17β-estradiol	ER agonist	SPE,RIA	0.92 ± 0.11 ng/L	2.8 ± 0.35 ng/L	8.9 ± 1.0 ng/L	27.9 ± 2.9 ng/L	84.3 ± 7.1 ng/L
4-t-octylphenol (MED)	ER agonist	LC or GC	6.2 ± 0.6 μg/L	12.5 ± 1.5 μg/L	25.1 ± 2.6 μg/L	51.1 ± 5.6 μg/L	102.3 ± 8.5 μg/L
4-t-octylphenol (NIES)	ER agonist	none	6.25 μg/L	12.5 μg/L	25 μg/L	50 μg/L	100 μg/L
o,p’-DDT	ER agonist	GC-MS	0.03 ± 0.004 μg/L	0.07 ± 0.01 μg/L	0.22 ± 0.04 μg/L	0.69 ± 0.17 μg/L	1.90 ± 0.43 μg/L
4-chloro-3-methylphenol	ER agonist	HPLC	20.7 ± 4.6 μg/L	43.3 ± 14.1 μg/L	88.0 ± 27.7 μg/L	165.1 ± 22.1 μg/L	344.7 ± 74.4 μg/L
tamoxifen	ER antagonist	GC-MS	1.32 ± 0.19 μg/L	2.51 ± 0.28 μg/L	5.08 ± 0.65 μg/L	10.17 ± 1.34 μg/L	20.44 ± 2.58 μg/L
17β-trenbolone	AR agonist	SPE, HPLC	2.2 ± 0.5 ng/L	5.1 ± 1.2 ng/L	12.9 ± 2.7 ng/L	31.7 ± 4.8 ng/L	84.2 ± 13.0 ng/L
vinclozolin	AR antagonist	HPLC	17.3 ± 1.6 μg/L	33.2 ± 3.4 μg/L	69.5 ± 6.2 μg/L	136.3 ± 17.6 μg/L	253.3 ± 58.7 μg/L
prochloraz	Steroidogenesis inhibitor	HPLC	5.3 ± 1.1 μg/L	9.2 ± 1.7 μg/L	17.5 ± 3.1 μg/L	25.0 ± 3.8 μg/L	41.1 ± 3.4 μg/L

ER = estrogen receptor; AR = androgen receptor; SPE = solid phase extraction; RIA = radioimmunoassay; LC = liquid chromatography; GC = gas chromatography; MS = mass spectrometry; HPLC = high-performance liquid chromatography