Table 1. Formulation Characteristics of Three Batches of VEGF-Loaded Microspheres Prepared with a 50:50 Blend Ratio of Polymer A and B, per Batch and Average Values ± SD for Each Formulation Characteristic.
batch | yield [%] | average size [μm]; CV [%] | feed loading [wt %] | actual loading [wt %]a | loading efficiency [%] |
---|---|---|---|---|---|
1 | 74 | 51; 15 | 1.00 | 0.72 | 72 |
2 | 73 | 52; 30 | 0.93 | 0.90 | 96 |
3 | 65 | 44; 17 | 1.13 | 0.76 | 67 |
average ± SD | 71 ± 5 | 49 ± 4 | 1.02 ± 0.10 | 0.79 ± 0.09 | 78 ± 16 |
Loading of VEGF in microspheres was determined after destruction of loaded microspheres in DMSO and NaOH. VEGF content was quantified by BCA protein assay.