Table 3. Composition of the Water and Oil Phases Used for the Preparation of Microspheres.
|
target VEGF loading |
|||
---|---|---|---|---|
formulation parameters | Placebo | 0.2 wt % | 1.0 wt % | |
w1-phase | VEGF concentration | n.a.a | 2 mg/mL | 15–20 mg/mL |
o-phase | polymer concentration in DCM | 10 wt % | 10 wt % | 15–20 wt % |
ratio VEGF/polymer | n.a. | 1:500 | 1:100 | |
w2-phase | continuous phase | 0.4 wt % PVA, 5 wt % NaCl | 0.4 wt % PVA, 5 wt % NaCl | 4 wt % PVA, 5 wt % NaCl |
n.a. = not applicable.