Table 3. Bacterial Strains, Plasmids, and Primers Used in This Study.

bacterial strains, plasmids, or primers		characteristics or sequence	source or reference
bacterial strains	L. interrogans serovar Copenhageni strain Fiocruz L1-130	wild-type	ICMR, Port Blair
	L. interrogans serovar Lai
	L. biflexa serovar Patoc
	E. coli DH5α	supE44 ΔlacU169 (ϕ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96thi-1relA1	Novagen
	E. coli BL21 (DE3)	F–ompT hsdSb (rBmB–) gal(λ c I 857 ind 1 Sam7 nin5 lacUVt7gene 1)dcm (DE3)
plasmids	pTZ57R/T	linearized TA vector with 3′-ddT overhangs for TA cloning of PCR products	Thermo Scientific
	pET23a	bacterial vector for expression of C-terminally His6-tagged proteins	Novagen
primers	clpP1F	5′CTAGCTAGCATGGCGTAATCCCGTATGTG3′(NheI)	this work
	clpP1R	5′CCGCTCGAGTTCAGTTTGTTTACGATCGATCT3′(XhoI)
	clpP2F	5′CTAGCTAGCATGCCAGAAACAGAAAAATCG3′(NheI)
	clpP2R	5′CCGCTCGAGATTAAAATCGATTTTAGTAGCGAG3′(XhoI)
	clpXF	5′CTAGCTAGCTTGGCTAAGAAAACACCGG3′(NheI)
	clpXR	5′CCGCTCGAGAGCAATCTTAGATTCTTTTTTGAG3′(XhoI)
	clpP1(S98A)F	5′TCAGGCTTCTgctCTATGGCGGC3′
	clpP1(S98A)R	5′CCTAAACAAAGAGTTCTTACATC3′
	clpP2(S97A)F	5′AATGGCTGCTgctCTATGGGTTCTG3′
	clpP2(S97A)R	5′CCCATACAAACCGTGTGAAC3′