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. 2019 Jul 31;4(7):12969–12977. doi: 10.1021/acsomega.9b00973

Figure 4.

Figure 4

3D culture: side view of muscle cells and motor neurons cocultured in matrigel. The muscle cells are usually allowed to differentiate into muscle fibers in the matrigel before the motor neurons are added on top of them and differentiated to form neuron-muscle contacts.