Figure 1.

Adult bird sexing by detecting a fragment of the XhoI repetitive sequence using modified double-quenched probes. Fluorescent signal as a function of the number of PCR cycles, measured using (a) the MMB system and (b) qPCR system. The total number of samples tested was 12. Shown are five adult females (solid red), five adult males (dashed green), a negative control without target DNA (dashed violet), and a negative control without Taq polymerase enzyme (dotted orange). The error bars for the male and female samples represent the standard deviation of five independent samples (n = 5). The error bars for the negative controls represent the standard deviation of three measurements (n = 3).