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Sex determination of chick embryos using conventional PCR. A PCR test, targeting a female-specific nonrepetitive HUR0417 sequence, is used as a gold standard to determine the chick embryos’ sex. All of the amplified sequences were at the expected size (642 bp). Samples with a band at the expected size were considered as female. No nonspecific amplification was observed in any of the control samples. A total of 16 samples are presented (out of 25 samples that were tested).
