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. 2019 Jul 8;4(7):11749–11755. doi: 10.1021/acsomega.9b01071

Figure 3.

Figure 3

Sex determination of chick embryos using qPCR and the MMB system. A total of 12 male and 13 female samples were tested using (a) qPCR and (b) the MMB system. Using qPCR, to positively identify all female samples, at least 13 cycles are required. Using the MMB system, after eight cycles, the receiver operating characteristic cutoff value allows sex determination with 100% sensitivity (all of the female samples are identified as females) and 100% specificity (all of the male samples are identified as males).