Table 1. Probe and Primers Used for the Development of the Assay.
| oligonucleotide | sequence (5′–3′) |
|---|---|
| XhoI forward primer | 5′-CTGCACTTCCTTCCCGAAA-3′ |
| XhoI reverse primer | 5′-CGGCTGAAAGGTGGTACTT-3′ |
| ZEN probea | 5′-/Alex532N//iBiodT/CGCTTCACT/ZEN/CACAAAGCACGC/3IABkFQ/-3′ |
| HUR0417 forward primer | 5′-ACACTATGTTTTCTGCCCGC-3′ |
| HUR0417 reverse primer | 5′-CTGAGGGTTGACCTTTCCAA-3′ |
a
ZEN probe includes two black quenchers: ZEN quencher (proprietary to IDT) at the ninth nucleotide and an Iowa Black Quencher (3IABkFQ) at 3′ end of the probe.