Fig 1. NBI design.

(a) NBI includes an entrance atrium monitored by a motion sensor and a nest compartment monitored by a thermal and IR camera (illuminated by an IR LED). (b) Path of mouse to the nest compartment. (c) Representative image captured from thermal camera showing low, 80-×-60-pixel resolution. (d) Sheathing to protect wires from mice. (e) Mill used to cut plastic frame. (f) NBI placed in cage showing single-board computer (Raspberry Pi) to execute imaging programs and record data (note this current NBI system is powered by a wall plug). IR, infrared; LED, light-emitting diode; NBI, nesting box imager.