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. 2019 Jul 24;15(7):e1008292. doi: 10.1371/journal.pgen.1008292

Fig 4. Seeds undergoing controlled deterioration treatment (CDT) increase PA levels and enhance phyA-mediated seed germination.

Fig 4

(A) Histograms show germination percentages with WT and phyA seeds of different ages exposed to a FR/48h/FR assay. Three technical repetitions of the germination percentage of 50–65 seeds were used for SD. (B) Free PA (Put, Spd and Spm) levels in CDT-exposed WT seeds exposed to a FR/48h/FR assay. PA levels were measured 24 h after the second FR pulse. Three technical repetitions were used. (C) Germination percentages of WT seeds shown in (B) after exposure to a FR, FR/48h/FR or FR/R assay. For each repetition, the germination percentage of 50–65 seeds is shown by a red dot. The average germination percentage for the three repetitions is represented by a horizontal black bar. Statistical treatment as in Fig 1B. Experiments in (B) and (C) were repeated with two biological seed batch samples, which provided similar results (S7B, S8B and S8C Figs).