Fig. 2.

a A typical workflow at the Mistral Beamline. Cells are grown on top of TEM grids and vitrified. Once frozen, the grids are checked with visible light. Using a regular fluorescence microscope setup and a cryo-stage, the quality of the sample (cell density, flatness of the grid, vitrification success) is checked and potential areas of interest are selected. If available, high-resolution fluorescence data can be collected as well. After fluorescence imaging, the samples are transferred to the TXM chamber. An on-line fluorescence microscope is used to re-locate the areas previously imaged, and tilt-series are collected from the previously imaged cells. b A mosaic image overlaid with the fluorescent signal coming from a mitochondrial stain obtained by cryo structured illumination microscopy (SIM). c One slice of the reconstructed volume from the yellow box in b. Most of the signal is coming from the mitochondrial membranes, which is the target of the dye. Scale bar: B, 10 μm; C, 2 μm