Table 3.
Expression of antimicrobial peptides in pancreatic tissue and duodenal mucosa in 10 non-diabetic organ donors aged between 1 and 27 years and expression data of the encoding gene in previously published data.
| Molecule | IHC Positivity score# Exocrine tissue |
IHC Positivity score# Endocrine Tissue |
IHC Positivity score# Duodenum |
Staining patterns | Encoding gene and its expression in previously published datasets*, Median (range) |
|---|---|---|---|---|---|
| Cathelicidin | -/+ | -/+/++ | - | Varies between donors, one donor completely negative. Exocrine parenchyma generally negative, singular cells and cell clusters positive. Islet parenchyma negative, singular positive cells. Duodenal mucosa negative. |
CAMP LCM islets: 0 (0–0.13) Isolated islets: 0.1 (0–0.5) Isolated exocrine: 0.5 (0.2–0.5) |
| Defensin Alpha-1 | +/++/+++ | - | -/+ | Exocrine acinar cells largely positive but varies between donors. Some donors show negative acinar cells. Islet parenchyma negative. Dodenal mucosa positive in a few donors. |
DEFA1B LCM islets 0 (0–0.26) Isolated islets: 4.4 (0.0–8.4) Isolated exocrine: 9.4 (8.8–11.5) |
| Defensin Beta-1 | - | - | - | - |
DEFB1 LCM islets: 4.4 (0–244) Isolated islets: 20.4 (13–85) Isolated exocrine: 215 (206–235) |
| Defensin Beta-2 | ++ | ++/+++ | ++/+++/++++ | Exocrine acinar cells largely positive. Islet parenchyma strongly positive Duodenal mucosa shows varying positivity between donors. |
DEFB4A LCM islets: 0 (0–1.13) Isolated islets: 0 (0–3.7) Isolated exocrine: 0.2 (0–7.4) |
| Defensin Beta-3 | + | - | Exocrine parenchyma negative. Islet cell cytoplasms partly positive. Duodenal mucosa negative. |
DEFB103A LCM islets: 0 (0–0.87) Isolated islets: 0 (0–0) Isolated exocrine: 0 (0–0.2) |
|
| Defensin Beta-4 | - | - | - | - |
DEFB4B LCM islets: 0 (0–0.13) Isolated islets: 0 (0–0.6) Isolated exocrine: 0 (0–1.3) |
| GP2 | +/++/+++/++++ | - | - | Exocrine acinar cells shows varying degrees of positivity between cells. Islet parenchyma negative. Duodenal mucosa negative. |
GP2 LCM islets: 382 (46–5774) Isolated islets: 56 (33–172) Isolated exocrine: 966 (603–1515) |
| Defensin Alpha-4 | - | +/++ | +/++/+++ | Exocrine parenchyma negative. Islet cells generally positive. Variation between donors. Duodenal mucosa shows varying positivity between donors |
DEFA4 LCM islets: 0 (0–0) Isolated islets: 0 (0–0.4) Isolated exocrine: 0 (0–0) |
| REG3A | -/+/++ | -/+/++ | +/++/+++ | Two completely negative donors. Exocrine parenchyma shows singular positive cells and clusters in most donors, completely negative in some. Several donors show positive Islet cells. Duodenal mucosa shows varying degrees of positivity. |
REG3A LCM islets: 14 (0.15–1356) Isolated islets: 47 (5.3–1762) Isolated exocrine: 800 (717– 1564) |
#;IHC staining was graded from (–, negative) to (++++, strongly positive)
*Data from previously published datasets17 of expression of the encoding gene in islets extracted by laser capture microdissection, isolated islets and isolated exocrine cell clusters. Data from LCM islets had been acquired by AmpliSeq are expressed as normalized counts whereas data from isolated islets and exocrine tissue are expressed as fpkm (fragments per kilo base per million mapped reads).