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Percent distribution of tweets belonging to the ten themes in the negative sentiment category.
| Theme | Distribution of tweets, % |
| Brain defects | 12 |
| Neurological effects | 12 |
| Initial reports | 11 |
| Zika impact | 11 |
| Fetal effects | 11 |
| Zika abnormalities | 10 |
| Ultrasounds | 10 |
| Guillain-Barré syndrome | 9 |
| Zika repercussion | 9 |
| Dengue association | 5 |
