Figure 2.

IFA-induced lymphangiogenesis is altered in A2a-deficient mice. WT and A2a-deficient mice (10 mice per group) were challenge with two i.p injections of IFA (day 0 and day 10) and sacrificed 10 days after the second injection to evaluate LEC infiltration in diaphragms. (A) Begnin lymphangioma (circled in yellow) on the diaphragm of WT and A2aKO animals (day 20). (B) Quantitation of lymphangioma weights after dissection. Absolute number of Lyve1⁺/CD11b^neg conventional LEC (C), CD11b⁺/Lyve1⁺ myeloid-like LEC (M-LEC) (D) and lyve1^neg/CD11b⁺ myeloid cells (E) per diaphragm were determined by flow cytometry. (F) Representative FACS plots illustrating the data obtained in the panels C, D and E are shown (FMO: Fluorescence Minus One). Means ± standard errors are shown. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 using a Student t test. Results are representative of 2 independent experiments.