Figure 6.
A2a signaling on both hematopoietic and non-hematopoietic cells promotes sentinel lymph node metastasis. WT and A2a-deficient mice were lethally irradiated, adoptively transferred with WT or A2a-deficient bone marrow, and injected 8 weeks later with 2.5 × 105 B16-CD73+ melanoma cells in the foot pad. Tumor metastasis to the draining popliteal was evaluated 30 days later by measuring (A) popliteal lymph node weights, (B) metastatic incidence and (C) GFP fluorescence on homogenized tumor-draining lymph nodes. Means ± standard errors are shown. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 using a Student t test. Results are representative of one experiment with 15-20 mice per group.