VEGF-C levels are reduced in A2a-deficient sentinel lymph nodes.
WT and A2a-deficient mice were injected with 2.5 × 105 B16-CD73+ melanoma cells in the foot pad and two weeks later tumor-draining lymph nodes and contralateral lymph nodes were collected. Primary tumors and lymph node were homogenized, centrifuged and supernatants were assayed for VEGF levels by ELISA. (a) Fold-increase in VEGF-A, C and D in tumor-draining lymph nodes (TdLN) over matched contraleral lymph nodes (cLN). (b) Levels of VEGF-A, C and D in tumors. Means ± standard errors are shown. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 using a Student t test. Results of 2 independent experiments with 10 mice per group were pooled.