Figure 8.

The number of VEGF-C secreting B cells is reduced in A2a-deficient sentinel lymph nodes.

WT and A2a-deficient mice were injected with 2.5 × 10⁵ B16-CD73+ melanoma cells in the foot pad. (a) Two weeks later, tumor-draining (TdLN) and contralateral (cLN) lymph nodes were collected and assayed for VEGF-C levels by ELISA and for flow cytometry for (b) CD45.2⁺/CD19⁺ B cells, and (c) CD45.2⁺/CD11b⁺/F4/80⁺ macrophages. (d) Representative section of a WT sentinel lymph node showing B220 and VEGF-C co-staining (scale bar = 20 microns). (e) VEGF-C production by WT and A2aKO purified lymph node B cells. Means ± standard errors are shown. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 using a Student t test. Results of 2 independent experiments with 10 mice per group were pooled.