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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Dig Liver Dis. 2019 Apr 16;51(8):1154–1163. doi: 10.1016/j.dld.2019.03.020

Figure 2.

Figure 2.

Effect of alcohol on the mRNA expression of the hepatic lipid and fibrogenic genes in SphK2−/− mice. WT) and SphK2−/− mice (male and female, 20 weeks old) were fed Lieber-DeCarli control diet or 5% alcohol diet for 60 days. Total liver RNA was isolated, and the mRNA levels of target genes were determined using quantitative RT-PCR and normalized to Hprt1 as an internal control. (A) The mRNA levels of key genes in bile acid synthesis and lipid metabolism. (B) The mRNA levels of fibrogenic genes. Results are represented as mean ± SE from each group (n = 7). Statistical significance relative to the WT group, *P < 0.05.