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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Dig Liver Dis. 2019 Apr 16;51(8):1154–1163. doi: 10.1016/j.dld.2019.03.020

Figure 5.

Figure 5.

The hepatic SphK2 expression is downregulated in the patients of alcoholic cirrhosis and hepatocellular carcinoma (HCC). (A) Total RNA was isolated from human liver samples from normal control, alcoholic cirrhosis or HCC patients (both male and female). The mRNA levels of SphK2 were determined using quantitative RT-PCR and normalized to HPRT1 as an internal control. Results are represented as mean ± SE from each group (n = 8). (B) The protein levels of SphK2 were determined by Western Blot analysis. Representative immunoblot images of SphK2 and actin are shown. (C) The relative protein level of SPHK2 was determined by normalizing to ACTIN as a loading control. Results are represented as mean ± SE from each group (n = 3–6). Statistical significance relative to the normal control group, *P < 0.05.