Skip to main content
. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Cancer Lett. 2019 Jul 6;461:1–9. doi: 10.1016/j.canlet.2019.07.002

Figure 3. Tumor-associated macrophage (TAM) repolarization using miR-125b in HA-PEI nanoparticles.

Figure 3.

Female C57BL/6 mice were injected with ID8 cells on day 1. On day 10, the mice were injected intraperitoneally with either HA-PEI-scrambled miR or HA-PEI-miR-125b at 1 mg/kg dose every alternate day for 3 days and peritoneal cavity was lavaged with 1X PBS on day 16, for peritoneal cells collection and analysis by FACS for (A) CD11b+, F4/80+ macrophages, (B) CD206+ M2 macrophages and (C) CD80+ M1 macrophages (D) Ratio of CD206/CD80. Expression of (E) iNOS and (F) Arg-1 and (G) iNOS/Arg-1 ratio tumor-associated macrophages treated with HA-PEI nanoparticle formulations. qPCR was used for quantification of gene expression level with β-actin as the internal control. N=4, Data is analyzed by One-way ANOVA.