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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Cancer Lett. 2019 Jul 6;461:1–9. doi: 10.1016/j.canlet.2019.07.002

Figure 4. Analysis of immune cells from the peritoneal lavage fluid upon miR-125b transfection.

Figure 4.

Female C57BL/6 mice were injected with ID8 cells on day 1. On day 10th mice were injected intraperitoneally with either HA-PEI-scrambled miR or HA-PEI-miR-125b at 1 mg/kg dose every alternate day for 3 days and peritoneal cavity was lavaged with 1X PBS of day 16th for peritoneal cells collection and analysis by FACS for (A) CD45+ (B) PD1+ gated on CD45+ cells, (C) CD11b+, CD11c+ dendritic cells, (D) GFP+ ID8 cells and (E) PD-L1+ with GFP+ cells. N=4, Data is analyzed by One-way ANOVA.