. Author manuscript; available in PMC: 2020 Sep 1.

Published in final edited form as: Hum Immunol. 2019 Feb 5;80(9):633–643. doi: 10.1016/j.humimm.2019.01.010

Table 4A:

Pairwise combination

Sample	Relationship	HLA-DPA1	HLA-DPB1	Informative	HLA-DPA1~ HLA-DPB1
4A1	child	DPA101:03:01:02+ DPA101:03:01:04	DPB104:01:01:01+ DPB1104:01	False	DPA101:03:01:04~ DPB104:01:01:01+ DPA101:03:01:02~ DPB1104:01
4A2	child	DPA101:03:01:02+ DPA101:03:01:04	DPB104:01:01:01+ DPB1104:01	False	DPA101:03:01:04~ DPB104:01:01:01+ DPA101:03:01:02~ DPB1104:01
4A3	mother	DPA101:03:01:02+ DPA101:03:01:04	DPB104:01:01:01+ DPB1104:01		*DPA101:03:01:04~ DPB104:01:01:01+ DPA101:03:01:02~ DPB1*104:01
4A4	father	DPA101:03:01:02+ DPA101:03:01:04	DPB104:01:01:01+ DPB1104:01		DPA101:03:01:04~ DPB104:01:01:01+ DPA101:03:01:02~ DPB1104:01
4A5	child	DPA101:03:01:04+ DPA101:03:01:04	DPB104:01:01:01+ DPB104:01:01:01	True	*DPA101:03:01:04~ DPB104:01:01:01+ DPA101:03:01:04~ DPB1*04:01:01:01

HLA-DPA1 and HLA-DPB1 columns show the original genotypes presented in GL String format. The prefix “HLA-” is omitted from gene names in this table. “Informative” column indicates whether the child genotypes are informative (True) or not (False) for building haplotypes using allele segregation. Maternal (4A3), paternal (4A4) and two children’s (4A1 and 4A2) genotypes of these loci are completely identical. There are two possible haplotype combinations for mother (4A3), father (4A4) and two children (4A1 and 4A2), thus it is not feasible to separate the children’s HLA alleles into unique parental alleles. These loci for these family members contain uninformative genotypes. However, only one haplotype combination can be built using the third child 4A5’s genotype and the parents’. The haplotypes are shown in bold and underlined letters. Each haplotype for 4A5 is inherited from the parents. When one haplotype is built for the parents, it is possible to build the second haplotype for each parent by subtracting the first haplotype from the parental genotypes.