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. 2019 Jul 26;11(7):431–444. doi: 10.4252/wjsc.v11.i7.431

Table 1.

Summary of in vitro neuronal reprogramming studies discussed in this review with details of transcription factors used and neuronal characteristics

Species Cell of origin Target cell Transcription factor(s) Neuronal characteristic Year and ref.
Mouse Embryonic fibroblast iN Ascl1 Neuronal morphology Neuronal markers Functional electrophysiology 2014[12]
Human Fetal fibroblast iN ASCL1 Neuronal morphology Neuronal markers 2014[12]
Human Fibroblast iN ASCL1, SOX2 and NGN2 Neuronal morphology Neuronal markers Neuronal gene expression profile Functional electrophysiology 2015[14]
Mouse Embryonic fibroblast iN Ascl1 Simple neuronal morphology Neuronal markers 2010[25]
Mouse Embryonic fibroblast iN (mostly GABAergic and glutamatergic neurons) Brn2, Myt1l, Zic1, Olig2, and Ascl1 Neuronal morphology Neuronal markers Functional electrophysiology Synaptic maturation 2010[25]
Mouse Embryonic fibroblast iN (mostly excitatory neurons) Brn2, Myt1l, and Ascl1 Neuronal morphology Neuronal markers Functional electrophysiology Synaptic maturation 2010[25]
Mouse Adult tail tip fibroblast iN (mostly excitatory neurons) Brn2, Myt1l, and Ascl1 Neuronal morphology Neuronal markers Functional electrophysiology Synaptic maturation 2010[25]
Human Fibroblast iN (mostly dopaminergic neurons) BRN2, MYT1, ASCL1, LMX1A and FOXA4 Neuronal markers Functional electrophysiology 2011[26]
Mouse Embryonic fibroblast iN (mostly dopaminergic neurons) Mash1, Nurr1 and Lmx1a Neuronal markers Neuronal gene expression profile Neuronal epigenetic reactivation Functional electrophysiology 2011[33]
Human Adult fibroblast iN (mostly dopaminergic neurons) MASH1, NURR1 and LMX1A Neuronal morphology Neuronal markers Neuronal gene expression profile Functional electrophysiology 2011[33]
Human Fibroblast iN (mostly dopaminergic neurons) ASCL1, NGN2, SOX2, NURR1 and PITX3 Neuronal morphology Neuronal markers Neuronal gene expression profile Functional electrophysiology 2012[35]
Mouse Embryonic fibroblast Induced sensory neurons Brn3a and Ngn1 or Ngn2 Neuronal gene expression profile Functional electrophysiology Synaptic maturation 2015[38]
Human Adult fibroblast Induced sensory neurons BRN3A and NGN1 or NGN2 Neuronal morphology Neuronal markers Neuronal morphology Neuronal markers Functional electrophysiology 2015[38]
Mouse Embryonic fibroblast Induced nociceptors Ascl1, Myt1l, Ngn1, Isl2, and Klf7 Neuronal morphology Neuronal markers Neuronal gene expression profile Functional electrophysiology Synaptic maturation 2014[43]
Human Fibroblast Induced nociceptors ASCL1, MYT1L, NGN1, ISL2 and KLF7 Neuronal markers Functional electrophysiology 2014[43]
Human Iris cells Photoreceptor- like cells Crx, Rx and Neurod1 Neuronal morphology Neuronal markers Neuronal gene expression profile Functional light electrophysiology 2012[47]
Human Dermal fibroblast Photoreceptor- like cells CRX, RAX, NEUROD1 and OTX2 Neuronal markers Neuronal gene expression profile Functional light electrophysiology 2014[48]
Mouse Müller glia iN (mostly retinal glia- like neurons) Neurog2 Neuronal morphology Neuronal markers Functional electrophysiology Neuronal gene expression profile 2018[56]
Mouse Müller glia iN (mostly retinal neurons) Ascl1 Neuronal morphology Neuronal markers Functional electrophysiology Neuronal gene expression profile 2018[56]
Mouse Cerebellum astroglia iN (mostly glutamatergic neurons) Neurog2 Neuronal morphology Neuronal markers Synaptic maturation Functional electrophysiology 2017[57]
Mouse Cerebellum astroglia iN (mostly glutamatergic neurons) Ascl1 Neuronal morphology Neuronal markers Synaptic maturation Functional electrophysiology 2017[57]

iN: Induced neurons.