Figure 5.

The DTT-induced potentiation of the NMDAR-synaptic response is not due to zinc chelation. The panels A-C illustrate the time course for the NMDAR-fEPSP slope; each point represents the mean (±SEM), normalized to the baseline (dashed line). (A) The arrow indicates the time of bath application of DTT (0.5 mM) in presence of ZnCl₂ (1 µM). (B) The arrow indicates the time of bath application of ZX1 (100 µM). (C) The last ten min of NMDAR-fEPSP slope recording in presence of ZX1 was renormalized and DTT was added (arrow). (D) Bar graph represents the mean (+SEM) percent change in NMDAR-mediated fEPSP during the last 5 min of recording, in response to Zn²⁺ plus DTT (open bar, n = 3/3 slices/animals), ZX1 alone (gray bar, n = 11/6 slices/animals,) and ZX1 plus DTT (filled bar, n = 11/6 slices/animals). Asterisks indicate significant potentiation relative to baseline.