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. 2019 Jul 29;11(14):5258–5275. doi: 10.18632/aging.102123

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Copyright © 2019 Bezold et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of IL-10 after glycation. THP-1 macrophages were glycated with 1 mM MGO or treated with 10% AGE-FCS and polarized in M1 or M2 phenotype. Expression of IL-10 was quantified using qPCR (A). Data was normalized to untreated control cells. Graph shows average mean of relative mRNA expression + SD of 3 independent experiments. Protein secretion of IL-10 was quantified in the cell supernatant using cytometric bead array (B). Graph shows average mean of IL-10 concentration (in pg/mL) + SD of 3 independent experiments.