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. 2019 Jul 29;11(14):5258–5275. doi: 10.18632/aging.102123

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Copyright © 2019 Bezold et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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ROS production after glycation. THP-1 macrophages (M0) were treated with different MGO concentrations, 10% AGE-FCS or different concentrations of H₂O₂ for up to 60 min. Production of intracellular ROS was determined using fluorescent probe H₂DCFDA and measurement of fluorescence intensity. Basic measurement represents fluorescence intensity after loading of the cells with H₂DCFDA but without addition of any treatment. Shown is one representative graph of 3 independent measurements. Data represents average mean + SD of 5 technical replicates.