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Albumin binding kinetics of the organometallic ions RhCp* (a) and RuCym (b) followed by UV–Vis spectrometry, and calculated molar absorbance (ε) spectra of the HSA-free and HSA-bound forms of RhCp* and RuCym (c). Inset shows the absorption changes at 350 nm for RhCp* (empty circle) and at 390 nm for RuCym (filled diamond), respectively. Spectra depicted here are subtracted by the spectrum of HSA [cRh = 20 µM and cHSA = 10 µM (a); cRu = 100 µM and cHSA = 33 µM (b); PBS’; 25 °C]
