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. 2019 Jul 30;10:1762. doi: 10.3389/fmicb.2019.01762

FIGURE 3.

FIGURE 3

Expression of PrtR in the ΔKH-S1 mutant. (A) Fragments of the prtR promoter region fused with the prtR-His or a promoterless lacZ gene. (B) Protein levels of PrtR-His in PAK and the ΔKH-S1 mutant carrying the PprtR-prtR-His on the bacterial chromosome. The bacterial cells were grown to an OD600 of 1.0, and then incubated with or without 0.06 μg/ml ciprofloxacin for 1 h. The PrtR-His levels were determined by Western blotting. RpoA was used as the loading control. (C) PAK containing an empty vector or the prtR overexpression plasmid was grown to an OD600 of 1.0 and treated with 0.16 μg/ml ciprofloxacin for 6 h. At indicated time points, the bacterial survival rate was determined by serial dilution and plating. ∗∗∗p < 0.001 by Student’s t-test.