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. 2019 Jul 30;10:1762. doi: 10.3389/fmicb.2019.01762

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Copyright © 2019 Fan, Chen, Li, Pan, Fu, Ren, Chen, Bai, Jin, Cheng, Jin and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Translational regulation of prtR by the PNPase. (A) Structures of the 6 × His-tagged prtR fusions. P_BAD-43-prtR-His and P_BAD-15-prtR-His represent 6 × His-tagged prtR driven by the P_BAD promoter with 43 and 15 bp of the 5′-UTR sequence of the prtR gene, respectively. The prtR open reading frame was replaced by a gfp gene, resulting in P_BAD-43-gfp and P_BAD-15-gfp. The potential ribosome binding site (RBS) was shown in bold underlined letters. Strains containing the prtR-His or gfp expression plasmid were grown to an OD₆₀₀ of 1.0 and then induced with 0.2% arabinose for 1.5 h. Protein levels of PrtR (B,C) and GFP (D,E) were determined by Western blotting. RpoA was used as the loading control.