TABLE 1.
Bacterial strains, plasmids and primers used in this study.
| Strain/Plasmid/Primer | Description | Source/Purpose |
| P. aeruginosa | ||
| PAK | Wild type strain of Pseudomonas aeruginosa | David Bradley |
| ΔKH-S1 | PAK with pnp (KH and S1) deletion | Chen et al., 2016 |
| ΔKH-S1 /Tn7T-pnp | PAKΔKH-S1 with pnp inserted on chromosome with mini-Tn7T insertion; | Chen et al., 2016 |
| PAKΔPA0614 | PAK deleted of PA0614 | This study |
| PAKΔPA0629 | PAK deleted of PA0629 | This study |
| PAKΔprtN | PAK deleted of prtN | This study |
| ΔKH-S1ΔPA0614 | PAKΔKH-S1 deleted of PA0614 | This study |
| ΔKH-S1ΔPA0629 | PAKΔKH-S1 deleted of PA0629 | This study |
| ΔKH-S1ΔprtN | PAKΔKH-S1 deleted of prtN | This study |
| PAK/pMMB67EH | PAK containing plasmid pMMB67EH | This study |
| ΔKH-S1/pMMB67EH | PAKΔKH-S1 containing plasmid pMMB67EH | This study |
| PAK/pMMB67EH-prtR | PAK containing plasmid pMMB67EH-prtR | Chen et al., 2017 |
| Plasmid | ||
| pEX18Tc | Gene replacement vector; Tcr, oriT+, sacB+ | Hoang et al., 1998 |
| pUC18T-mini-Tn7T-Tc | mini-Tn7 base vector from insertion into chromosome attTn7 site; Tcr | Choi and Schweizer, 2006 |
| pUC18T-mini-Tn7T-PprtR-lacZ | prtR promoter of PAK fused to promoterless lacZ on pUC18T-mini-Tn7T | Sun et al., 2014 |
| pMMB67EH | Expression vector with tac promoter;Apr | Furste et al., 1986 |
| pUCP20 (no promoter) | Escherichia-Pseudomonas shuttle vector; no promoter; Ampr | This study |
| pUCP20(no promoter) -pRkaraRed(43)-PrtR-His | 6 × His-tagged PrtR driven by the PBAD promoter with 43 bp of the 5′-UTR sequence on pUCP20(no promoter) | This study |
| pUCP20(no promoter) -pRkaraRed(15)-PrtR-His | 6 × His-tagged PrtR driven by the PBAD promoter with 15 bp of the 5′-UTR sequence on pUCP20(no promoter) | This study |
| pUCP20(no promoter) -pRkaraRed(43)-GFP | GFP driven by the PBAD promoter with 43 bp of the 5′-UTR sequence on pUCP20(no promoter) | This study |
| pUCP20(no promoter) -pRkaraRed(15)-GFP | GFP driven by the PBAD promoter with 15 bp of the 5′-UTR sequence on pUCP20(no promoter) | This study |
| Primer | Sequence (5′→3′) | Function |
| PA0636-RT-S | TGGAAGACCCGGCAGAAG | RT-PCR |
| PA0636-RT-AS | CGTTGAGCTTGGACAGATCCT | RT-PCR |
| PA0614-RT-S | CGCTGCCTGCCAAGGA | RT-PCR |
| PA0614-RT-AS | ATCAGTACCCAGAGCGGCATT | RT-PCR |
| PA0629-RT-S | GTGGAGAACCTCAATTACAG | RT-PCR |
| PA0629-RT-AS | TAGGTGTTGTCGGCAATC | RT-PCR |
| prtR-RT-S | GATGCGCAACCTGAAGCA | RT-PCR |
| prtR-RT-AS | TGAATGGTGTTCTGCGAAACC | RT-PCR |
| prtN-RT-S | CGACGATAGCCACAAG | RT-PCR |
| prtN-RT-AS | GGATGCGATGCTGTC | RT-PCR |
| lexA-RT-S | AATCCCGCCTTCTTCAAT | RT-PCR |
| lexA-RT-AS | AATGCCGATGTCCTTCAT | RT-PCR |
| recA-RT-S | ATATCAAGAACGCCAACT | RT-PCR |
| recA-RT-AS | TAGAACTTCAGTGCGTTA | RT-PCR |
| BamHI-PprtR-lacZ-S# | CGCGGATCC GAGCCAGGACCAGTTCGTTGGC | Transcriptional fusion |
| HindIII-lacZ-AS | ATTATAAAGCTT TTATTTTTGACACCAGACCAACTGG | Transcriptional fusion |
| SacI-PBAD-S | CCAAGAGCTC TTATGACAACTTGACGGC | Translational fusion |
| HindIII-prtR-AS | ATTATAAAGCTT TCAGTGGTGGTGGTGGTGGTGACCTCCCC GCACCAGGGACGGGCCGC | Translational fusion |
| XhoI- prtR(43)-GFP S | CCGCTCGAG TAGGCTCTTTACAGAAAATCCATCGGTCTGTAGA TTGCCGAGCATGAGTAAAGGAGAAGAACTTTTCACTG | Translational fusion |
| XhoI- prtR(15)-GFP S | CCGCTCGAG TGTAGATTGCCGAGCATGAGTAAAGGAGAAGAA CTTTTCACTG | Translational fusion |
| HindIII –GFP-AS | CCCAAGCTT TTATTTGTATAGTTCATCCATGCCATG | Translational fusion |
#The endonuclease cutting sites are underlined.