FIGURE 1.

Schematic illustration of the experimental design. (A) Mice were randomly allocated to sham or stroke group. At day 7 post-stroke mice were euthanized and brains were sliced into 2 mm thin sections using a matrix device. Brain samples were divided into two different groups. The brain slides from the first group (TTC+) were stained with 2% TTC at 37°C for 10 min, then further dissected into infarct (IF), thalamus (Th), and hippocampus (Hippo) and frozen at −80°C. The slices from the second group (TTC−) were dissected and immediately frozen at −80°C. (B) Top panel: images of TTC+ mouse brain slices after photothrombotic stroke. After the brain have been sliced, we placed the 2 mm section on a grid mat. Once we have taken a picture of one side (rostral, row 1) of the brain, we flipped the sections and take a picture of the other side (caudal, row 2). Bottom panel: schematic illustration showing the location of the examined regions (IF, Th, and Hippo) (left). Quantification of the IF volume in TTC+ stroke mice (right).