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. 2019 Jul 30;10:1776. doi: 10.3389/fimmu.2019.01776

Figure 4.

Figure 4

Construction of HCMV-based vaccines expressing E6 peptide fused to the C-terminus of HCMV IE1 or HCMV UL83. The HLA-A2-binding peptide E629−38 (TIHDIILECV) derived from the E6 protein of HPV-16 was fused with an AA-linker (AATIHDIILECV) to the C-terminus of (A) HCMV UL123 (IE1) (RVTB40ΔUS11_E6peptideIE1) or (B) HCMV UL83 (RVTB40ΔUS11_E6peptideUL83). (C) In addition, a recombinant virus expressing both the E6/E7 fusion protein inserted into UL83 and the E6-peptide fused to the C-terminus of HCMV IE1 was generated (RVTB40ΔUS11_ E6/E7intoUL83_E6peptideUL83).