Figure 6.

NFAT-driven EGFP expression in reporter cell lines stimulated by infected GBM cells. 5 × 10⁴ GBM cells (LN18, U343, or U251 cells) were infected with HCMV-based vaccines (MOI of 5). After 2 and 4 days, respectively, infected cells were co-cultured with HPV E6-specific reporter cells (left graphs) and HCMV pp65-specific reporter cells (right graphs), respectively, for 24 h at a ratio 2:1. Subsequently, EGFP expression of reporter cells was determined by FACS analysis. Uninfected cells (Mock) and cells infected with RVTB40ΔUS11 (Control) were also included in this type of analysis. Stimulation of reporter cells is given as percentage of maximal peptide stimulation, i.e., stimulation of reporter cells incubated with peptide pulsed cells. Results are derived from three technical replicates; error bars represent the mean ± SEM. ^****P < 0.0001; ^***P < 0.001; ^**P < 0.01; ^*P < 0.05, one-way ANOVA test.