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. 2019 Jul 30;10:1790. doi: 10.3389/fimmu.2019.01790

Figure 3.

Figure 3

Overexpression of PDE4A restores early proliferation and cytokine secretion in peripheral blood T-cells under suppression by PGE2 or adenosine-receptor agonists. FACS-sorted control-vector transduced (squares) or PDE4A transduced (circles) human peripheral blood CD4+ (left panels) or CD8+ T-cells (right panels) were activated with agonistic anti-CD3/anti-CD28 antibodies in the absence or presence of the indicated concentrations of PGE2 (A) or the A2A-R agonist CGS21680 (B). After 72 h, cells were labeled with [3H]-thymidine for another 18 h and thymidine incorporation was measured. Mean values ± SD from triplicate cultures from one representative donor (n = 6) are depicted. (C) 24 h (IL-2) or 72 h (IFN-γ, TNF-α) after activation supernatants were harvested and concentrations of the indicated cytokines were measured by ELISA. Mean values ± SD from triplicate cultures from one representative donor (n = 7) are depicted. n.s., not significant; ***p < 0.001.