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. 2019 Jul 30;10:856. doi: 10.3389/fphar.2019.00856

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Copyright © 2019 Rong, Wan, Fan, Liu, Sun, Huo, Zhang, Li, Xie, Wang and Sun

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MTT analysis of the effect of amentoflavone on viability of BV2 microglial cells. (A) Structure of amentoflavone. (B) Viability of BV2 microglial cells treated with amentoflavone in graded concentrations (0, 50, 100, 250, 500, 750, or 1000 nM) was measured by MTT assay (one-way ANOVA followed by Dunnett’s test). (C) Viability of BV2 microglial cells treated with amentoflavone in graded concentrations (5, 10, 25, 50, 75, 100, 125, and 150 µM) was measured by MTT assay (one-way ANOVA followed by Dunnett’s test). Error bars represent mean ± s.d. Compared to control group, *** represents p < 0.001. All experiments were performed in triplicate. AF, amentoflavone.