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. 2019 Jul 30;10:950. doi: 10.3389/fpls.2019.00950

FIGURE 1.

FIGURE 1

Geitlerinema has two putative ethylene receptors. A BLAST search of the Geitlerinema PCC 7105 genome reveals that it has two genes, GeiEtr1 and GeiEtr2, that are predicted to encode proteins with an ethylene binding domain. (A) Alignment of the predicted amino acid sequences of the ethylene-binding domains of GeiEtr1 and GeiEtr2 with the ethylene-binding domains of the receptors from Synechocystis sp. PCC 6803 (Syn) and Arabidopsis thaliana (At). Shaded residues are conserved in all eight sequences. Black circles above the sequences mark amino acids required for ethylene binding to AtETR1. (B) Unrooted tree based on the amino acid sequences of the ethylene binding domains from panel A. The cladogram was generated using Clustal Omega with default settings and visualized using FigTree version 1.4.2. (C) Predicted domain structures of GeiEtr1 and GeiEtr2. Domain predictions were made using the Simple Modular Architecture Research Tool (SMART) http://smart.embl-heidelberg.de/ (Schultz et al., 1998; Letunic et al., 2012). (D) Transcript abundance of GeiEtr1 and GeiEtr2 in air versus 1000 nL L–1 ethylene were determined using qRT-PCR. Data were normalized to the levels of TruB and then to the level of each gene in air and represent the average ± SEM. P-values were determined using Student’s t-test.