FIGURE 5.

Specific ethylene binding. Ethylene-binding assays were conducted as detailed in the section “Materials and Methods” on (A) Geitlerinema cells, (B) Synechocystis cells, and (C) P. pastoris cells expressing the ethylene binding domain of SynEtr1 (SynEtr1[1-130]GST). Assays were carried out at the indicated ethylene concentrations of ¹⁴C-ethylene to determine total binding. Non-specific binding was determined using the same concentrations of ¹⁴C-ethylene in the presence of 1000-fold excess non-radioactive ethylene. Specific binding was then calculated by subtracting non-specific binding activity from total binding activity. Data are the average specific binding ± SD.