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. 2019 Jul 30;13:336. doi: 10.3389/fncel.2019.00336

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Copyright © 2019 Daviaud, Chevalier, Friedel and Zou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cellular distinction of different neural populations in response to HI. (A) IF images of representative 100 × 300 μm cortical columns labeled for the indicated markers at the indicated time points after hypoxia or control. White dashed lines demarcate VZ and outer layers (SVZ/CP). (B) Top graphs: quantifications of the percentage of DAPI⁺ cells across all cortical layers in radial columns that are positive for SOX2 at the indicated time points after hypoxia or control. Bottom graphs: quantifications of the number of SOX2⁺ cells in VZ-like layer at the indicated time points. Student’s t test, n = 9 organoids from three different batches. (C) Quantifications of the percentage of DAPI⁺ cells in cortical columns that are positive for FAM107A. Student’s t test, n = 9. (D) Quantifications of immunostaining intensity for DCX normalized to unit area (expressed as A.U.). Student’s t test, n = 9. (E) Quantifications of the percentage of DAPI⁺ cells across all cortical layers in radial columns that are positive for TBR2 at the indicated time points after hypoxia or control. (F) Top: quantifications of percentage of DAPI⁺ cells positive for CTIP2 in radial columns. Bottom: quantifications of the number of CTIP2⁺ cell in radial columns at 1 dpi. Student’s t test, n = 9. A.U., arbitrary unit. ^*p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; n.s., not-statistically significant.