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. 2019 Jul 30;13:336. doi: 10.3389/fncel.2019.00336

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Copyright © 2019 Daviaud, Chevalier, Friedel and Zou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of isochronic progenitor cohorts reveals vulnerable window and developmental stages affected by HI. (A) Timeline of experimental paradigm to analyze different isochronic cohorts (cohort 1 and 3) of neural precursors and their progeny that are birth-dated at different time points relative to transient HI using 30 min EdU or BrdU pulses followed by chase periods until analysis. (B) Left: Representative fluorescence images show abundance and spatial distribution of BrdU or EdU LRCs of isochronic cohort 1 or 3 at indicated time points during organoid development in control or after HI. White dashed lines demarcate VZ and SVZ/CP-like layers in 100 × 300 μm radial cortical columns. Right: Quantifications of BrdU⁺ or EdU⁺ LRCs in VZ or SVZ/CP layers. Two-way ANOVA followed by a Tukey post hoc test; n = 9 independent organoids from three different batches. ^*p < 0.05; ^∗∗p < 0.01; n.s., not-statistically significant.