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. 2019 Jul 30;13:336. doi: 10.3389/fncel.2019.00336

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Copyright © 2019 Daviaud, Chevalier, Friedel and Zou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Prolonged effect of transient HI on survival of differentiating population in SVZ. (A) Timeline of experimental paradigm to analyze isochronic cohort 1 and 3, birth-dated at D21 or D29 by 30 min EdU or BrdU pulse, respectively, and analyzed at D42 (14 dpi). The vulnerable windows for each cohort are depicted as green bars. (B,C) Representative IF images for CC3 and DCX in D42 cerebral organoids. Dashed white lines demarcate VZ and SVZ/CP layers. (B) For cohort 1 cells birth-dated at D21 (1 week before HI) and analyzed at D42 (14 dpi), colocalization of EdU, CC3, and DCX staining was observed in HI condition (white arrows), but rarely in control (white arrowheads point to no overlap). (C) For cohort 3 cells birth-dated at D29 and analyzed at D42 (14 dpi), no colocalization of BrdU, CC3 and DCX staining was observed in control (white arrowheads), but after HI, apoptotic LRCs with remnant of colocalized DCX and BrdU immunosignals were observed (white arrows). Enlarged images of area outlined by dashed orange box are shown on the right.