Figure 6.

HtrA1 is naturally present throughout the corneal stroma. Human corneal sections were cut from the epithelium (Epi) toward the endothelium (Endo) of 8-mm diameter corneal buttons (biological replicates, n = 4). Tissue sections were subjected to SDS-PAGE followed by immunoblotting against HtrA1. HtrA1 was more or less uniformly distributed throughout the corneal stroma and, hence, not recruited only upon amyloid formation. This result implies that TGFBIp degradation products containing LCD-linked mutations in the FAS1-4 domain can be turned over immediately once they are formed.