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. 2019 Jun 17;294(31):11944–11951. doi: 10.1074/jbc.RA119.008778

Figure 1.

Figure 1.

Selective deletion of IMP2 from mouse liver causes increased triglyceride deposition. A, relative liver IMP2 mRNA abundance during development. Livers were harvested from three WT male mice at the indicated times. The abundance of IMP2 mRNA was determined by QPCR, normalized to the level of 18S RNA, and divided by the IMP2 mRNA at postnatal day 21. B, abundance of IMP2 polypeptide in the livers of C57/Bl6j, Imp2ff, Alb-Cre+/−, and Imp2ff/Alb-Cre+/− mice. Livers were taken from three males of each genotype at 8 weeks age on the HFD. The numbers on the left in the blot in this and subsequent figures indicates the Mr in kilodaltons of nearby molecular mass markers. MEF, mouse embryonic fibroblast. C, liver triglyceride (TG) levels in Imp2ff and Imp2ff/Alb-Cre+/− mice. Male mice, 12 Imp2ff and 14 Imp2ff/Alb-Cre+/−, 30 weeks of age, on the HFD from 4 weeks; mean values are indicated in red. D and E, relative abundance of liver mRNAs concerned with neutral lipid synthesis (D) and catabolism (E). RNA was extracted from livers of four pairs of Imp2ff and Imp2ff/Alb-Cre+/− mice fed the HFD at 30 weeks of age, and the relative abundance of the mRNAs indicated was determined by QPCR. Oligonucleotides are listed in Table S1. F, immunoblot of Imp2ff and Imp2ff/Alb-Cre+/− liver extracts. HFD-fed male mice, 30 weeks of age. Antibodies are listed in Table S1. *, p < 0.05; **, p < 0.01.